Combined opto-impedimetric whole-cell biosensors  for environmental applications

Quang Thong Trinh; Dich Nguyen; Viet Phuong Trieu; Gerald Gerlach

doi:10.64032/mca.v29i4.330

Authors

Quang Thong Trinh Hanoi University of Science and Technology, Hanoi, Vietnam
Nguyen Quang Dich The Institute of Control and Automation Technology (ICEA), Hanoi University of Science and Technology (HUST), Hanoi, Vietnam
Viet Phuong Trieu Vietnam National Standard and Quality Institute (VSQI), Hanoi, Vietnam
Gerald Gerlach Institute of Solid State Electronics (IFE), Dresden University of Technology (TUD), Dresden, Germany

DOI:

https://doi.org/10.64032/mca.v29i4.330

Keywords:

whole cell sensor, PDMS microfluidics, fluorescent protein, Diclofenac

Abstract

Environmental pollution is the big concern of people in developing countries like Vietnam. In this scope, electrochemical sensors based on impedance technique have proved to be a promising method due to their portability, rapidity, sensitivity, low cost, ease of miniaturization, and label-free operation that can be used for on-the-spot detection. In this work, the design and fabrication of the whole-cell sensors for the environmental and food-safety applications were implemented. The sensor structure consists of a glass plate with two coated transparent ITO (indium tin oxide) microelectrode arrays and four-compartment micro-fluidic cell. The electrode structure is in the form of 24 inter-digitated fingers so-called comb-structure with a length of 11 mm. The living cells are integrated into sensor structure for the evaluation of use biological effect of analytic substance. The impedance measurement was combined with the optical detection of the fluorescence signal by using microfluidic structure. The sensor was tested for the detection of diclofenac concentrations between 10 and 100 μM in nutrient medium. The obtained results show the obvious changes in output signals for both of impedance and fluorescence characterization of the investigated samples.

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Author Biographies

Quang Thong Trinh, Hanoi University of Science and Technology, Hanoi, Vietnam

Quang Thong TRINH received MSc degree in materials science from International Trainning Institute for Materials Science (ITIMS) in 1999, and PhD degree in sensor technology & electrical engineering from Dresden University of Technology (TUD), Germany in 2006. From 2000 to date, he has been working for Faculty of Engineering Physics (FEP), Hanoi University of Science and Technology (HUST), Vietnam both as senior researcher and lecturer in physics. His current interests are development of electronic materials and devices, especially, MEMS-based sensors and acuators for trafic, environment, and bio-medical applications.

Viet Phuong Trieu, Vietnam National Standard and Quality Institute (VSQI), Hanoi, Vietnam

Phuong Viet TRIEU received his MSc degree in Control Engineering and Automation in 2007 and PhD degree in Measurment Engineering and Industrial Informatics in 2018 from Hanoi University of Science and Technology, Vietnam. Currently, he is the head of Vietnam National Standard and Quality Institute (VSQI). His research interest is development of sensors and measurements applied for environmental and industrial safety

Gerald Gerlach, Institute of Solid State Electronics (IFE), Dresden University of Technology (TUD), Dresden, Germany

Gerald GERLACH received MSc and PhD degrees in electrical engineering from Dresden University of Technology in 1983 and 1987, respectively. He worked in research and development in the field of sensors and measuring devices and sensors at several companies. In 1993, he became a professor at the Department of Electrical Engineering at Dresden University of Technology. From 1996 to date, he has been the head of the Institute for Solid-State Electronics. Prof. Gerlach had served as Deputy Dean and Dean of the Electrical Engineering Department at TU Dresden, respectively between 1994 to 2000. He also had been vice president of the German Society for Measurement and Automation Technology (GMA) as well as member of the Presidency of the VDE (German Association of Engineers in Electrical Engineering, Electronics, Information Technology) since 2002. His research focused on development of sensor and semiconductor technology, simulation and modelling of micromechanical devices and the development of solid-state sensors.